|First Appearance (Barney and the Rocko Squad)||Little Einsteins: Our Huge Adventure|
|Barney and the Rocko Squad Portrayer||Natalia Wojcik|
- — Annie's usual response in Little Einsteins.
Annie is a 4-year-old blonde girl who loves to sing. She is Leo's beloved little sister, the youngest Little Einstein, the only character who's piloted Rocket alone and who had change her clothes. She owns a silver microphone with orange music notes that she won in a song contest after Big Jet ripped her music, which was previously seen in the Little Einsteins episode Annie, Get Your Microphone. In the 2nd season of Little Einsteins, the color of her shirt was changed from green to light-blue or white, her jumper was changed from blue to pink and her shoes were changed from pink to blue.
Natalia Wojcik is Annie's voice actress.